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Updated: May 15, 2026

Fluorescence Live-cell Imaging of the Complete Vegetative Cell Cycle of the Slow-growing Social Bacterium Myxococcus xanthus
Published on: June 20, 2018
Analysis of exopolysaccharides in Myxococcus xanthus using confocal laser scanning microscopy
Wei Hu1, Renate Lux, Wenyuan Shi
1School of Dentistry, University of California, Los Angeles, CA, USA.
Abstract:
Confocal laser scanning microscopy (CLSM) is a useful research tool to explore the exopolysaccharides (EPS) in bacterial biofilms. Here, we describe the analysis of different biofilms of Myxococcus xanthusformed in a modified chamber slide system with CLSM. In conjunction with several specific fluorescent probes, the EPS within M. xanthusbiofilms can be visualized and analyzed in situ.
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