Related Experiment Video
Updated: May 15, 2026

A High-throughput-compatible FRET-based Platform for Identification and Characterization of Botulinum Neurotoxin Light Chain Modulators
Published on: December 27, 2013
A pharmacodynamic comparison study of different botulinum toxin type A preparations
Sung-Beom Kim1, Bhupal Ban, Keun-Suk Jung
1Microbial Toxin Research Institute, Medytox Inc., Chungbuk, Korea.
Background:
Because more botulinum toxin (BoNT) preparations have become available worldwide, there is a clinical need to compare the pharmacologic profiles of these products.
Objective:
We compared three different preparations: onabotulinumtoxinA (ona-BoNT/A), abobotulinumtoxinA (abo-BoNT/A), and Neuronox (neu-BoNT/A), in a mouse model using a digit abduction scoring (DAS) assay.
Methods:
The efficacy, duration of effect, and safety margin of each preparation was determined after delivering a single injection to the right gastrocnemius (0-240 U/kg body weight of neu-BoNT/A or ona-BoNT/A; 0-600 Speywood Units/kg body weight of abo-BoNT/A).
Results:
Neu-BoNT/A (intramuscular (IM) median effective dose (ED(50) ) 11.2 ± 2.7 U/kg) and ona-BoNT/A (IM ED(50) 11.9 ± 2.4 U/kg) had similar effects in terms of muscle weakness at significantly lower doses than abo-BoNT/A (IM ED(50) 41.2 ± 2.4 U/kg; p < .001). The safety margin (ratio between IM ED(50) and IM median lethal dose (LD(50) )) of neu-BoNT/A (10.7 ± 2.6 U/kg) was also similar to that of ona-BoNT/A (10.3 ± 1.3 U/kg) but significantly higher than that of abo-BoNT/A (5.9 ± 0.4 U/kg; p < .02). Neu-BoNT/A and ona-BoNT/A also produced comparable patterns of DAS response and body weight recovery by day 29.
Conclusion:
Neu-BoNT/A and ona-BoNT/A may be interchangeable based on a simple dose ratio.
Related Concept Videos
Directly Acting Muscle Relaxants: Dantrolene and Botulinum Toxin
The binding of dantrolene to the RYR1...
Local Anesthetics: Differential Sensitivity of Nerve Fibers
Nondepolarizing (Competitive) Neuromuscular Blockers: Pharmacokinetics
Instead, they are transported by the blood to different tissues. Muscles with a greater blood supply (arteries) and blood flow receive more...
Depolarizing Blockers: Pharmocokinetics
Peripherally and Centrally Acting Muscle Relaxants: A Comparison
Centrally acting muscle relaxants can be further divided into spasmolytic and antispasmodic drugs. Spasmolytic drugs,...
Pharmacodynamic Responses: Different Types

