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Updated: May 15, 2026

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids
Published on: March 2, 2012
Effect of a low-conductivity zone on field-amplified sample stacking in microchip micellar electrokinetic
Kenji Sueyoshi1, Fumihiko Kitagawa, Koji Otsuka
1Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Nishikyo, Kyoto, Japan. sueyoshi@anchem.mc.kyoto-u.ac.jp
Abstract:
The effects of a low-conductivity (low-C) zone of an on-line sample preconcentration based on field-amplified sample stacking (FASS) under an acidic condition was investigated in microchip micellar electrokinetic chromatography (MCMEKC). By employing originally fabricated microchips with T- and cross-channel injectors on a single device, partial injection of the low-C zone and large-volume injection of a sample solution were demonstrated. As a typical result, the observed peak intensities of the fluorescent dyes were 1.5 - 2.0-fold enhanced by partial injection of the low-conductivity zone, as compared to those in FASS-MCMEKC without injecting the low-C zone; also, 30 - 40-fold enhancements of the detectability were achieved as compared to the conventional MCMEKC analyses. The resolutions of the hydrophobic analytes were also improved by introducing the low-C zone. The difference in the retention factors of model analytes in the high-C background and low-C zones was also estimated in order to clarify the effect of the low-C zone on the resolution and detectability in FASS-MCMEKC.
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