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Updated: May 15, 2026

11:24
Direct Protein Delivery to Mammalian Cells Using Cell-permeable Cys2-His2 Zinc-finger Domains
Published on: March 25, 2015
Femtomolar Zn(II) affinity of minimal zinc hook peptides--a promising small tag for protein engineering
Tomasz Kochańczyk1, Piotr Jakimowicz, Artur Krężel
1Laboratory of Chemical Biology, University of Wrocław, ul. Tamka 2, 50-137 Wrocław, Poland.
Abstract:
The minimal zinc hook peptide of Rad50 and its alanine mutants form highly stable Zn(II) complexes. These peptides were successfully used as a small, efficient tag for reversible Zn(II)-mediated protein homodimerization. The high stability, its biological consequences and potential applications in protein engineering are discussed.

