Related Experiment Video
Updated: May 15, 2026
09:38
Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry
Published on: June 26, 2019
Hepatoma-derived growth factor and nucleolin exist in the same ribonucleoprotein complex
Stephanie Bremer1, Katharina Klein, Angela Sedlmaier
1Institute of Biochemistry and Molecular Biology, University of Bonn, Nussallee 11, Bonn 53115, Germany.
BMC Biochemistry
|January 12, 2013
Summary
Hepatoma-derived growth factor (HDGF) and nucleolin form complexes dependent on specific bcl-2 mRNA. This interaction is crucial for HDGF
Area of Science:
- Molecular Biology
- Cancer Research
- Protein-RNA Interactions
Background:
- Hepatoma-derived growth factor (HDGF) is highly expressed in tumors and exhibits various biological activities.
- The molecular mechanisms and biological functions of HDGF in tumors remain largely unknown.
- Investigating HDGF interactions is crucial for understanding its role in cancer.
Purpose of the Study:
- To analyze the HDGF interactome using mass spectrometry.
- To characterize the interaction between HDGF and nucleolin.
- To elucidate the role of bcl-2 mRNA in HDGF/nucleolin complex formation.
Main Methods:
- Mass spectrometry to identify HDGF interacting proteins.
- Pull-down experiments to confirm HDGF and nucleolin interaction.
- Analysis of HDGF/nucleolin complex formation with varying bcl-2 mRNA constructs.
Main Results:
- Nucleolin was identified as the most abundant protein interacting with HDGF.
- HDGF influences nucleolin localization, causing its redistribution to the cytoplasm.
- HDGF/nucleolin complex formation is dependent on bcl-2 mRNA, specifically requiring its coding sequence and UTRs.
Conclusions:
- HDGF and nucleolin form nucleoprotein complexes regulated by specific mRNAs.
- The formation of these complexes is dependent on the presence of bcl-2 mRNA.
- Further research is needed to identify other components and the precise nature of these RNA-dependent complexes.
More Related Videos
Related Concept Videos
02:55The Nucleolus
The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
02:55The Nucleolus
The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
02:31Nuclear Export of mRNA
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
02:31Nuclear Export of mRNA
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
02:50Conjugated Proteins
Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
03:03mTOR Signaling and Cancer Progression
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...