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Published on: February 19, 2016
Expression pattern of protein kinase Cδ during mouse embryogenesis
Sergio Carracedo1, Ursula Braun, Michael Leitges
1The Biotechnology Centre of Oslo, University of Oslo, Gaustadalleen 21, Oslo, Norway. s.c.huroz@biotek.uio.no
Background:
The members of the protein kinase C (PKC) family consist of serine/threonine kinases classified according to their regulatory domain. Those that belong to the novel PKC subfamily, such as PKCδ, are dependent on diacylglycerol but not Calcium when considering their catalytic activity. Although several studies have shown the importance of PKCδ in different cellular events in health and disease, the overall in vivo distribution of this PKC isoform during development is still lacking. Through Lac Z and antibody staining procedures, we show here the in vivo expression of PKCδ during mouse embryogenesis.
Results:
Ganglia were the domains with most prominent expression of PKCδ in most of the stages analysed, although PKCδ could also be detected in heart and somites at earlier stages, and cartilage primordium and skin among other sites in older embryos.
Conclusions:
The strong expression of PKCδ in ganglia during murine development shown in this study suggests a significant role of this isoform as well as redundancy with other PKCs within the nervous system, since PKCδ deficient mice develop normally.
Insights
Protein Kinase C delta (PKCδ) is highly expressed in developing mouse ganglia. This suggests a key role for PKCδ in nervous system development, despite normal development in PKCδ-deficient mice.
Area of Science:
- Developmental Biology
- Molecular Biology
- Neuroscience
Background:
- Protein Kinase C (PKC) family comprises serine/threonine kinases.
- Novel PKC subfamily members, like PKCδ, require diacylglycerol but not calcium for activity.
- PKCδ's role in cellular events is known, but its developmental distribution in vivo is unclear.
Purpose of the Study:
- To investigate the in vivo spatiotemporal expression of PKCδ during mouse embryogenesis.
Main Methods:
- Utilized Lac Z staining to track PKCδ expression patterns.
- Employed antibody staining for protein localization.
- Analyzed expression throughout various stages of mouse development.
Main Results:
- PKCδ showed prominent expression in developing ganglia across most analyzed stages.
- Early embryonic expression was also detected in the heart and somites.
- Later embryonic stages revealed PKCδ in cartilage primordium, skin, and other tissues.
Conclusions:
- Strong PKCδ expression in ganglia during murine development indicates a significant role in nervous system formation.
- The normal development of PKCδ-deficient mice suggests functional redundancy with other PKC isoforms in the nervous system.
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