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Combinational biosynthesis of phycocyanobilin using genetically-engineered Escherichia coli
Baosheng Ge1, Yan Li, Haixiang Sun
1Center for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao 266580, People's Republic of China.
Abstract:
Genes of the key enzymes for phycocyanobilin (PCB) biosynthesis were cloned into E. coli and combinationally expressed to produce phycocyanobilin, with autologous heme as substrate. Culture conditions were optimized to achieve ~3 mg PCB/l. A protocol for the purification of recombinant phycocyanobilin was established using solvent extraction combined with chromatography, which resulted in a final yield of ~0.3 mg PCB/l with a purity >95 %. Recombinant phycocyanobilin could scavenge hydroxyl radicals with an EC50 of 0.1 μM.
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