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Related Concept Videos

Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
From DNA to Protein03:06

From DNA to Protein

The flow of genetic information in cells from DNA to mRNA to protein is described by the central dogma, which states that genes specify the sequence of mRNAs, which in turn specify the sequence of amino acids making up all proteins. The decoding of one molecule to another is performed by specific proteins and RNAs. Because the information stored in DNA is so central to cellular function, it makes intuitive sense that the cell would make mRNA copies of this information for protein synthesis...
DNA as a Genetic Template02:05

DNA as a Genetic Template

Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
Nucleic Acid Structure01:25

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA has a double-helix structure. The...
The Central Dogma01:20

The Central Dogma

The central dogma explains the flow of genetic information from DNA nucleotides to the amino acid sequence of proteins.
RNA is the Missing Link Between DNA and Proteins
In the early 1900s, scientists discovered that DNA stores all the information needed for cellular functions and that proteins perform most of these functions. However, the mechanisms of converting genetic information into functional proteins remained unknown for many years. Initially, it was believed that a single gene is...

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DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition
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DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition

Published on: February 9, 2024

Single-stranded nucleic acid recognition: is there a code after all?

Antoine Cléry1, Julien Boudet1, Frédéric H-T Allain1

  • 1Institute of Molecular Biology and Biophysics, ETH Zürich, Switzerland.

Structure (London, England : 1993)
|January 15, 2013
PubMed
Summary
This summary is machine-generated.

Single-stranded DNA binding proteins with OB-fold structures were analyzed. Their co-adaptability with DNA sequences reveals insights into protein-DNA interactions.

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Area of Science:

  • Structural biology
  • Molecular biology
  • Biochemistry

Background:

  • Proteins interacting with single-stranded nucleic acids are vital for cellular processes.
  • Structural studies are key to elucidating the functions of these proteins.

Discussion:

  • Dickey and colleagues present high-resolution structures of an OB-fold protein bound to various single-stranded DNA (ssDNA) sequences.
  • The study highlights the dynamic and adaptable nature of protein-ssDNA interactions.

Key Insights:

  • Reveals a remarkable co-adaptability between the OB-fold protein and different ssDNA sequences.
  • Provides atomic-level understanding of how these proteins recognize and bind ssDNA.

Outlook:

  • Further structural and functional studies can illuminate the roles of these proteins in DNA replication, repair, and recombination.
  • Understanding these interactions may aid in the design of novel therapeutic agents targeting nucleic acid-binding proteins.