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Updated: May 15, 2026

Evaluation of Intracellular Location of Reactive Oxygen Species in Solea Senegalensis Spermatozoa
Published on: March 11, 2018
Cell membrane proteins from oviductal epithelial cell line protect human spermatozoa from oxidative damage
Venus W Huang1, Weie Zhao, Cheuk-Lun Lee
1Department of Obstetrics and Gynecology, University of Hong Kong, Queen Mary Hospital, Hong Kong, PR China.
Objective:
To study the potential protective action in vitro of oviductal epithelial cell membrane proteins against oxidative damage in human spermatozoa.
Design:
Prospective in vitro study.
Setting:
University research laboratory and infertility clinic.
Patient(S):
Semen from men attending the infertility clinic at the Queen Mary Hospital with normal semen parameters (World Health Organization, 2010).
Intervention(S):
We studied the effect of oviductal epithelial cell membrane proteins on the sperm functions and endogenous antioxidant enzyme activities.
Main Outcome Measure(S):
Sperm motility, lipid peroxidation, DNA fragmentation, intracellular reactive oxygen species (ROS) level, superoxide dismutase, and glutathione peroxidase activities.
Result(S):
Oviductal epithelial cell membrane proteins bind to the human spermatozoa and protect them from ROS-induced damages in terms of sperm motility, membrane integrity, DNA integrity, and intracellular ROS level. Spermatozoa-oviduct epithelial cell interaction also enhances the antioxidant defenses in spermatozoa.
Conclusion(S):
Our results demonstrated the protective effects of spermatozoon-oviductal epithelial cell interaction against oxidative stress in human spermatozoa. The results enhance our understanding of the protective mechanism of oviduct on sperm functions.
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Oogenesis
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