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Stromal Cell Isolation From Hematopoietic Organs
Published on: January 26, 2024
Mesenchymal stromal/stem cells markers in the human bone marrow
Valeria Rasini1, Massimo Dominici, Torsten Kluba
1Department of Oncology, Hematology and Respiratory Diseases, University Hospital of Modena and Reggio Emilia, Modena, Italy.
Cytotherapy
|January 15, 2013
Summary
Human bone marrow contains distinct progenitor cells expressing markers similar to cultured mesenchymal stromal/stem cells (MSCs). This finding supports MSCs
Area of Science:
- Stem cell biology
- Regenerative medicine
- Hematopoiesis
Background:
- Mesenchymal stromal/stem cells (MSCs) are crucial for regenerative therapies.
- The in vivo phenotype of bone marrow (BM)-MSCs remains poorly understood.
- It is unclear if ex vivo MSC markers reflect an in vivo counterpart.
Purpose of the Study:
- To investigate the in vivo phenotype of BM-MSCs.
- To characterize progenitor cells within their natural BM microenvironment.
- To identify distinct cellular populations expressing progenitor cell markers in human trabecular bone.
Main Methods:
- Multi-parametric immunohistochemistry on human trabecular bone biopsy specimens.
- Analysis of cell morphology and micro-anatomic localization.
- Assessment of MSC-associated antigen expression patterns.
Main Results:
- In vivo expression of key BM-MSC antigens (CD10, CD73, CD140b, CD146, GD2, CD271) was observed.
- Distinct progenitor cell populations expressing pluripotency markers (Oct4, Nanog, SSEA-4) were identified.
- An overlap in antigen expression between ex vivo expanded MSCs and in vivo cells was noted.
Conclusions:
- The bone marrow harbors identifiable and distinct progenitor cells.
- These progenitor cells exhibit overlapping phenotypes with ex vivo MSCs.
- The findings support the use of BM-derived progenitor cells in regenerative medicine.
