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Botulinic toxin detection by counterimmunoelectrophoresis
L Nuñez1, E A Amato de Lagarde, M d'Aquino
1Orientación Higiene y Sanidad, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina.
Zentralblatt Fur Bakteriologie : International Journal of Medical Microbiology
|March 1, 1990
Abstract:
A counterimmunoelectrophoretic (CIE) technique was developed to detect botulinic toxin type A, and the method was compared with the mouse bioassay. A 100 LD50 concentration was detected within 2 h. Crossed reactions were observed with antitoxins of types B and F. As regards other Clostridium species, precipitin lines were seen between C. sporogenes and antitoxin type A. Foodstuffs artificially contaminated with C. botulinum type A were assayed by means of CIE and mouse bioassay, without recording interference by substances normally contained in foodstuffs.