Monitoring angiogenesis using a human compatible calibration for broadband near-infrared spectroscopy
Runze Yang1, Qiong Zhang, Ying Wu
1University of Calgary, Department of Radiology, Calgary, Alberta, Canada.
Journal of Biomedical Optics
|January 15, 2013
Summary
This study shows that a new calibration method for broadband near-infrared spectroscopy (bNIRS) can noninvasively monitor angiogenesis by measuring cerebral blood volume (CBV) in animal models, making it suitable for human use.
Area of Science:
- Physiology
- Biomedical Engineering
Background:
- Angiogenesis, the formation of new blood vessels, is crucial in various conditions like cancer and diabetes.
- Cerebral blood volume (CBV) measured by broadband near-infrared spectroscopy (bNIRS) can indicate angiogenesis.
- Previous bNIRS methods for studying angiogenesis in animal models required patient-unsuitable calibration techniques.
Purpose of the Study:
- To determine if angiogenesis can be detected using a calibration method suitable for human application.
- To quantify changes in CBV as a marker of angiogenesis in a rat cortex model.
Main Methods:
- Rats were acclimated to a simulated high-altitude environment (370 mmHg) for three weeks.
- Cerebral blood volume (CBV) was measured using broadband near-infrared spectroscopy (bNIRS) before and after hypoxia acclimation.
- A control group of rats was housed under normal pressure conditions.
Main Results:
- The acclimation group showed a significant increase in mean CBV from 3.49%±0.43% to 4.76%±0.29%.
- The control group exhibited no significant changes in CBV (3.28%±0.75% to 3.09%±0.48%).
- This indicates successful monitoring of angiogenesis via CBV changes.
Conclusions:
- Angiogenesis can be monitored noninvasively over time using bNIRS.
- The developed calibration method is compatible with human use.
- This less stressful approach advances animal studies of angiogenesis.
