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Correlation of peroxisome proliferator-activated receptor (PPAR-γ) mRNA expression with Pro12Ala polymorphism in

Rym Berhouma1, Soumaya Kouidhi, Myriam Ammar

  • 1Laboratory of Genetics, Immunology and Human Pathologies, Faculty of Sciences of Tunis, University of El Manar 1, 2092, Tunis, Tunisia. berhoumar@gmail.com

Biochemical Genetics
|January 15, 2013
PubMed

Insights

Obese individuals show higher PPARγ mRNA expression and a greater frequency of the Pro/Pro genotype for the PPARγ Pro12Ala polymorphism, indicating a link to obesity risk.

Area of Science:

  • Genetics
  • Metabolism
  • Endocrinology

Background:

  • Peroxisome proliferator-activated receptor gamma (PPARγ) plays a crucial role in adipogenesis and metabolic regulation.
  • The Pro12Ala polymorphism in the PPARγ2 gene is a common variant studied for its association with obesity and related metabolic traits.

Purpose of the Study:

  • To investigate the correlation between PPARγ mRNA expression in subcutaneous adipose tissue and the Pro12Ala PPARγ2 polymorphism in the context of obesity.
  • To determine the association of specific PPARγ genotypes with obesity risk in the Tunisian population.

Main Methods:

  • Quantitative analysis of PPARγ mRNA expression in subcutaneous adipose tissue from obese and nonobese subjects.
  • Genotyping of the Pro12Ala polymorphism in the PPARγ2 gene using established methods.
  • Statistical analysis to compare gene expression levels and genotype frequencies between groups.

Main Results:

  • PPARγ mRNA expression was significantly higher in subcutaneous adipose tissue of obese subjects compared to nonobese controls (P < 0.05).
  • Obese subjects exhibited a significantly higher frequency of the Pro/Pro genotype (90.5%) compared to nonobese controls (79.5%) for the Pro12Ala polymorphism (P = 0.03).
  • The Pro12 allele was associated with increased PPARγ mRNA expression in subcutaneous adipocytes.

Conclusions:

  • The Pro12Ala polymorphism, particularly the Pro/Pro genotype, is associated with an increased risk of obesity in the Tunisian population.
  • The Pro12 allele of PPARγ is linked to PPARγ mRNA overexpression in subcutaneous adipose tissue.
  • The PPARγ Pro12Ala variant may contribute to variations in PPARγ mRNA expression, body mass index, and insulin sensitivity in the general population.

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