Related Experiment Video
Updated: May 15, 2026

Applications of Liquid-Chromatography Tandem Mass Spectrometry in Natural Products Research: Tropane Alkaloids as a Case Study
Published on: March 8, 2024
Review: Recent application of chiral liquid chromatography-tandem mass spectrometric methods for enantiomeric
Yanfang Nie1, Xiumei Liu, Xinmei Yang
1School of Pharmaceutical Sciences and Center for Pharmaceutical Research & Drug Delivery Systems, Shandong University, Jinan, Shandong 250012, China.
Abstract:
The technology of analyzing the pharmacological and toxicological properties of chiral drugs has become more commonly utilized since the knowledge of the biological actions of enantiomers has been gradually acquired during the last few decades. This work reviews the applications of chiral liquid chromatography coupled to tandem mass spectrometry (LC-MS) for the analysis of chiral pharmaceuticals since 2005. The enantioselective determinations in pharmaceutical and biomedical fields with LC-MS are classified based on three mobile phase modes, including normal phase, reversed phase and polar organic solvent, in terms of their compatibility with various ionization sources and specific applications.
Related Concept Videos
Tandem Mass Spectrometry
Chirality in Nature
High-Performance Liquid Chromatography: Types of Detectors
Racemic Mixtures and the Resolution of Enantiomers
Peptide Identification Using Tandem Mass Spectrometry
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Principles Of Column Chromatography
