Trophic effects of basic fibroblast growth factor on fetal rat hypothalamic cells: interactions with insulin-like...

The existence of different growth factors within a single brain region suggests that developing brain cells are exposed to a variety of trophic factors throughout neurogenesis. Cooperative interactions between growth factors are known to orchestrate growth and differentiation of various cell types. We explored the possibility that two growth factors may interact in promoting in vitro growth in fetal hypothalamic cells. We found that basic fibroblast growth factor (b-FGF) exerts trophic effects on primary mixed hypothalamic cell cultures, on enriched hypothalamic neuronal cultures, and on hypothalamic glial cultures. In addition, b-FGF increased the growth rate of two virally transformed hypothalamic cell lines. Since insulin-like growth factor I (IGF-I) also promotes growth of rat hypothalamic cells in vitro, we examined the combined effects of b-FGF and IGF-I on hypothalamic cells. Significantly higher numbers of neurite-bearing cells were present in primary mixed hypothalamic cultures when b-FGF and IGF-I were added together than were added separately. The effect was additive. These results establish b-FGF as a putative hypothalamic neurotrophic factor and demonstrate potential coordinate interactions between IGF-I and b-FGF in stimulating the growth or survival of developing hypothalamic cells.