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Enzymatic variability among Brazilian Pythium insidiosum isolates
Régis A Zanette1, Laerte Ferreiro, Sydney H Alves
1Programa de Pós-Graduação em Ciências Veterinárias, Setor de Micologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Revista Iberoamericana De Micologia
|January 16, 2013
Summary
Extracellular enzyme activity in Pythium insidiosum varies among Brazilian isolates. Phosphohydrolases and ester hydrolases were common, indicating their role in oomycete virulence.
Area of Science:
- Microbiology
- Mycology
- Oomycete research
Background:
- Pythium insidiosum is an oomycete from the Stramenopila kingdom.
- Hydrolytic enzyme secretion is crucial for P. insidiosum virulence and infection initiation.
Purpose of the Study:
- To assess the extracellular enzymatic activity of 14 Brazilian P. insidiosum isolates.
- To compare enzymatic profiles with a standard strain (ATCC 58637) using the API-ZYM System.
Main Methods:
- Culturing P. insidiosum zoospores in RPMI 1640 broth.
- Inoculating API-ZYM strips with 65 μL of the liquid phase for enzymatic analysis.
Main Results:
- Observed variations in enzymatic activities across different P. insidiosum isolates.
- Phosphohydrolases and ester hydrolases were consistently detected in all isolates.
- β-glucosidase was present in most isolates, while α-glucosidase and chymotrypsin were notably absent.
Conclusions:
- The observed enzymatic profile discrepancies among Brazilian P. insidiosum isolates highlight phenotypic variations.
- These variations may correlate with differences observed in susceptibility testing outcomes.
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