Related Experiment Video
Updated: May 15, 2026
12:40
Isolation and Expansion of Mesenchymal Stem/Stromal Cells Derived from Human Placenta Tissue
Published on: June 6, 2016
Expression and biological function of programmed death ligands in human placenta mesenchymal stem cells
Guoyan Wang1, Siying Zhang, Feifei Wang
1Department of Immunology, Binzhou Medical University, Shandong Province, Yantai 264003, P.R. China.
Cell Biology International
|January 16, 2013
Summary
Programmed death ligands 1 and 2 (PDL1 and PDL2) on human placenta mesenchymal stem cells (hPMSCs) regulate cell adhesion, migration, and immunosuppression. Modulating PDL1/PDL2 impacts hPMSC behavior and T-cell responses, offering insights for clinical applications.
Area of Science:
- Immunology
- Stem Cell Biology
- Regenerative Medicine
Background:
- Mesenchymal stem cells (MSCs) are crucial for tissue regeneration due to self-renewal, differentiation, and immunosuppression.
- Human placenta mesenchymal stem cells (hPMSCs) exhibit potent immunosuppressive capabilities, exceeding those of bone marrow MSCs.
- Programmed death ligands 1 and 2 (PDL1 and PDL2) are key regulators in immune responses.
Purpose of the Study:
- To investigate the roles of PDL1 and PDL2 in human placenta mesenchymal stem cells (hPMSCs).
- To determine the impact of PDL1 and PDL2 on hPMSC adhesion, migration, and immunosuppressive functions.
- To elucidate the effect of PDL1/PDL2 on T-cell activation and proliferation.
Main Methods:
- Utilized siRNA to knockdown PDL1 and/or PDL2 expression in hPMSCs.
- Assessed hPMSC adhesion and migration capabilities.
- Evaluated T-cell proliferation, cell cycle arrest, and CD69 expression following hPMSC interaction.
Main Results:
- High expression of PDL1 and PDL2 was observed in hPMSCs.
- PDL1/PDL2 knockdown significantly increased hPMSC adhesion but reduced migration.
- hPMSC-expressed PDL1 and PDL2 inhibited T-cell proliferation by arresting the cell cycle, without affecting CD69 expression.
Conclusions:
- PDL1 and PDL2 play critical roles in regulating hPMSC adhesion, migration, and immunosuppression.
- These findings highlight the functional significance of PDL1 and PDL2 in hPMSC immunomodulation.
- Understanding these roles may inform the clinical application of hPMSCs in cell-based therapies.
Related Concept Videos
01:19Mesenchymal Stem Cells
Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their access...
01:30Overview of Cell Death
Cell death is an essential process where the body gets rid of old or damaged cells. Cell proliferation and death need to be balanced, as an imbalance between the two may lead to cancer or autoimmune diseases.
Cell death was observed in the early 19th century, but there was no experimental evidence to prove it. In 1842, Carl Vogt first discovered cell death in a metamorphic toad; however, it was not termed ‘cell death.’ Scientists discovered different cell death pathways only in the 20th century...
Cell death was observed in the early 19th century, but there was no experimental evidence to prove it. In 1842, Carl Vogt first discovered cell death in a metamorphic toad; however, it was not termed ‘cell death.’ Scientists discovered different cell death pathways only in the 20th century...
01:01Regulation of Hematopoietic Stem Cells
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
01:22Cellular Injury V: Apoptosis and Autophagy
Cells respond to damage and stress through highly coordinated processes that decide whether they survive or undergo controlled self-destruction. Two major pathways involved in this regulation are apoptosis, a type of programmed cell death, and autophagy, a survival mechanism that helps cells adapt to adverse conditions.ApoptosisApoptosis removes aged or injured cells to maintain tissue balance. During this process, the cell shrinks, chromatin condenses and fragments, and membrane-bound...
01:17The Extrinsic Apoptotic Pathway
The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...