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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...

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Related Experiment Video

Updated: May 15, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Interlaboratory studies and initiatives developing standards for proteomics.

Alexander R Ivanov1, Christopher M Colangelo, Craig P Dufresne

  • 1Barnett Institute of Chemical and Biological Analysis, Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA 02115, USA. a.ivanov@neu.edu

Proteomics
|January 16, 2013
PubMed
Summary
This summary is machine-generated.

Proteomics research benefits from interlaboratory studies and standardization. A new performance standard for characterizing post-translational modifications (PTMs) by mass spectrometry (MS) was developed to address challenges in the field.

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Last Updated: May 15, 2026

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Area of Science:

  • Proteomics
  • Mass Spectrometry (MS)
  • Biomolecular Research

Background:

  • Proteomics is a rapidly evolving, interdisciplinary field utilizing diverse analytical techniques for biological research.
  • Challenges in proteomics research hinder progress in fundamental and applied biology.
  • Standardization initiatives are crucial for advancing the field and enabling reliable results.

Purpose of the Study:

  • To highlight the advantages of interlaboratory studies and standardization in proteomics.
  • To introduce a comprehensive performance standard for characterizing post-translational modifications (PTMs) using mass spectrometry (MS).
  • To discuss the development of this standard by the Association of Biomolecular Resource Facilities (ABRF) Proteomics Standards Research Group (sPRG).

Main Methods:

  • Review of existing proteomics research methodologies.
  • Discussion of interlaboratory study designs and their benefits.
  • Development and application of a novel performance standard for PTM characterization via MS.

Main Results:

  • Identification of key challenges in current proteomics research.
  • Demonstration of the value of collaborative efforts and standardization.
  • Successful development of a comprehensive performance standard for PTM analysis by MS.

Conclusions:

  • Interlaboratory studies and standardization are essential for overcoming proteomics challenges.
  • The ABRF sPRG's new performance standard offers a valuable tool for PTM characterization.
  • Adoption of such standards will enhance the reproducibility and reliability of proteomics research.