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[HPLC fingerprint of scrophulariae radix from southwest China]
Wen Zhou1, Lin Jiang, Xiao-Juan Wang
1Laboratory of Pharmacognosy and Natural Medicine Chemistry, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China. yews910@163.com
Objective:
To establish HPLC fingerprints of Scrophulariae Radix methanol extract from both the different southwest origin and market, provide evidence for its quality control.
Methods:
11 batches of samples analysis was performed on C18 column with gradient mobile phase of acetonitrile-0.4% acetic acid, the monitoring wavelength was at 260 nm with the flow rate of 1.0 mL/min, detective course was finished in 55 min.
Results:
Established the HPLC fingerprint common patterns by using harpagoside and cinnamic acid as the index components, and similarity with different origin rude drugs and pieces was compared. The similarity was above 0.901.
Conclusion:
This method contributes to the fast comprehensive evaluation of material quality and can be used specifically for standardized planting and quality control of Scrophulariae Radix.
