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Human urinary and renal alpha-L-fucosidases. A comparative study.
B Ricard1, R Roccetti, C Tassi
1Laboratoire de Chimie Biolgique, Université René Descartes, Paris, France.
The International Journal of Biochemistry
|January 1, 1990
Summary
Investigating alpha-L-fucosidase in human kidney and urine revealed distinct enzymatic forms. The urinary pattern closely matches the soluble form found in kidney tissue.
Area of Science:
- Biochemistry
- Human Physiology
- Enzymology
Background:
- Alpha-L-fucosidase is an enzyme crucial for cellular processes.
- Understanding its enzymatic forms in different human tissues is important for physiological studies.
Purpose of the Study:
- To investigate the enzymatic forms of alpha-L-fucosidase in human renal tissue and urine.
- To compare the isoenzymatic patterns between renal tissue extracts and urine.
Main Methods:
- Enzyme extraction from human renal tissue (soluble and detergent-solubilized).
- Chromatofocusing technique to separate isoenzymes.
- Analysis of enzymatic patterns in urine samples.
Main Results:
- Two distinct isoenzymatic patterns of alpha-L-fucosidase were identified in human renal tissue.
- The isoenzymatic pattern observed in urine was similar to the soluble extract from renal tissue.
Conclusions:
- Human renal alpha-L-fucosidase exhibits multiple enzymatic forms.
- Urinary alpha-L-fucosidase isoenzymes likely originate from the soluble fraction of renal tissue.