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Updated: May 15, 2026

Broth Microdilution In Vitro Screening: An Easy and Fast Method to Detect New Antifungal Compounds
Published on: February 14, 2018
A new compound from liquid fermentation broth of Armillaria mellea and the determination of its absolute
Yun-Chao Wang1, Yu-Wei Zhang, Li-Hua Zheng
1Institute of Genetics and Cytology, Northeast Normal University, Renmin Street 5268, Changchun 130024, China.
Abstract:
A new 2,5-diketopiperazine, (R)-2-(2-(furan-2-yl)-oxoethyl)-octahydropyrrolo[1,2-a]pyrazine-1,4-dione, and seven known compounds were isolated from the ethyl acetate extract of liquid fermentation broth of Armillaria mellea. The structures of the isolated compounds were established from NMR and HR-MS data. The absolute configuration of the new compound was established by comparing the experimental electronic circular dichroism (ECD) spectrum with the calculated ECD data.
