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The steroid-dependent regulatory element in the ovalbumin gene does not function as a typical steroid response
L A Schweers1, D E Frank, N L Weigel
1Department of Medicine, University of Minnesota, Minneapolis 55455.
The Journal of Biological Chemistry
|May 5, 1990
Summary
Steroid hormones regulate chicken ovalbumin gene transcription via a steroid-dependent regulatory element (SDRE). This element, along with a negative regulatory element, is crucial for gene induction, suggesting complex interactions are involved.
Area of Science:
- Molecular Biology
- Endocrinology
- Gene Regulation
Background:
- Chicken ovalbumin gene transcription is induced by steroid hormones.
- A steroid-dependent regulatory element (SDRE) was previously identified in the 5'-flanking region (-900 to -521).
Purpose of the Study:
- To precisely characterize the regulatory properties of the SDRE.
- To investigate the interaction between the SDRE and other regulatory elements.
Main Methods:
- Construction and transfection of ovalbumin-chloramphenicol acetyltransferase fusion genes.
- Site-directed mutagenesis within the SDRE region.
- Gel shift analyses using nuclear protein extracts.
Main Results:
- Mutations in the SDRE region (-900 to -732) abolished steroid hormone responsiveness.
- The SDRE required a negative regulatory element (-350 to -100) to confer steroid-dependent expression.
- Specific protein-DNA complexes involving the SDRE were observed in estrogen-treated oviduct cells.
Conclusions:
- Steroid-dependent ovalbumin gene induction involves complex interactions between the SDRE and a negative regulatory element.
- The SDRE does not function as a typical steroid response element independently.