Model for generation of large numbers of primary, inflammatory skin-derived neutrophils, and macrophages
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Updated: May 15, 2026

Development and Identification of a Novel Subpopulation of Human Neutrophil-derived Giant Phagocytes In Vitro
Published on: January 25, 2017
Dominik John1, Michael R Fischer, Esther von Stebut
1Department of Dermatology, University Medical Center, Johannes Gutenberg-University, Mainz, Germany.
Abstract:
Tissue macrophages and inflammatory neutrophils represent important cells of the innate immune system responsible for various important tasks, i.e., elimination of pathogens and/or granuloma formation. Isolation of large numbers of primary phagocytes is vital for research with these cells. Within this protocol, we present a strategy for isolation of large numbers of inflammatory neutrophils and macrophages from murine skin that allows for follow-up in vivo or in vitro studies.
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