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Differences in transcription patterns between induced pluripotent stem cells produced from the same germ layer are
Iryna Pirozhkova1, Ana Barat, Petr Dmitriev
1CNRS, Université Paris-Sud, UMR-8126, Institut de cancérologie Gustave Roussy, Villejuif, France.
Plos One
|January 18, 2013
Summary
Human myoblast-induced pluripotent stem cells (iPS) share similarities with fibroblast-derived iPS cells but show distinct gene expression patterns. These differences diminish upon differentiation into mesenchymal stem cells.
Area of Science:
- Stem cell biology
- Regenerative medicine
- Cellular reprogramming
Background:
- Induced pluripotent stem cells (iPS) are crucial for regenerative medicine.
- Understanding iPS cell origin impact on differentiation potential is vital.
- Limited data exists on iPS cells from tissues of the same germ layer.
Purpose of the Study:
- To compare human myoblast-derived iPS cells with those from fibroblasts.
- To investigate differences in gene expression and differentiation potential.
- To assess if germ layer origin influences iPS cell characteristics.
Main Methods:
- Generation of iPS cells from human primary myoblasts and fibroblasts via retroviral transduction (OCT3/4, SOX2, KLF4, MYC).
- In vitro cultivation and characterization of iPS cells, comparing morphology, stemness markers, and methylation patterns to embryonic stem cells.
- Formation of embryonic bodies and analysis of differentiation markers.
- Comparative analysis of transcription patterns between myoblast- and fibroblast-derived iPS cells and their differentiated mesenchymal stem cells.
Main Results:
- Myoblast- and fibroblast-derived iPS cells exhibited similar morphology, stemness marker expression, and methylation patterns to human embryonic stem cells.
- Both iPS cell types successfully formed embryonic bodies expressing endodermal, mesodermal, and ectodermal markers.
- Significant differences in gene expression were observed between myoblast- and fibroblast-derived iPS cells.
- These gene expression differences were reduced when comparing mesenchymal stem cells derived from both iPS cell types.
Conclusions:
- Human myoblast-derived iPS cells are comparable to fibroblast-derived iPS cells in fundamental stem cell characteristics.
- Distinct transcriptional profiles exist between iPS cells derived from different somatic tissues within the same germ layer.
- Mesenchymal stem cell differentiation mitigates some of the initial gene expression differences observed in the iPS cells.
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