Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Gas Chromatography: Types of Columns and Stationary Phases01:17

Gas Chromatography: Types of Columns and Stationary Phases

Gas chromatography (GC) relies on stationary phases to separate and analyze components in a sample. There are two main types of stationary phases: liquid and solid. Liquid stationary phases are non-volatile, thermally stable, and chemically inert liquids coated onto the column. Solid stationary phases are particles of adsorbent material, such as silica gel or molecular sieves.
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
Gas Chromatography: Introduction01:13

Gas Chromatography: Introduction

Gas chromatography (GC) is a technique for separating and analyzing volatile compounds in a sample. Its primary purpose is to identify and quantify components in complex mixtures, making it essential in fields such as environmental analysis, pharmaceuticals, and petrochemicals. GC is also called vapor-phase chromatography (VPC) or gas-liquid partition chromatography (GLPC).
In GC,  a sample is vaporized and mixed with an inert carrier gas (the mobile phase), which transports it through a column.
Gas Chromatography–Mass Spectrometry (GC–MS)01:14

Gas Chromatography–Mass Spectrometry (GC–MS)

Gas chromatography–mass spectrometry (GC–MS) is the combination of analytical techniques of gas chromatography and mass spectrometry in a single instrument for analyzing a mixture of compounds. The gas chromatograph separates the compounds in the mixture, and the mass spectrometer analyzes each compound separately to determine the molecular masses and molecular structures.
A gas chromatograph consists of a long, narrow capillary column with a polysiloxane coating on the inner wall. The coating...
Principles Of Column Chromatography01:13

Principles Of Column Chromatography

The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
Supercritical Fluid Chromatography01:18

Supercritical Fluid Chromatography

Supercritical fluid chromatography (SFC) provides a beneficial substitute for gas chromatography (GC) and liquid chromatography (LC) for certain samples because it merges the top attributes of both techniques. SFC allows the separation and analysis of compounds that GC or LC does not easily manage. These compounds are traditionally nonvolatile or thermally unstable, making GC unsuitable and lacking functional groups required for HPLC analysis.
SFC utilizes a supercritical fluid mobile phase,...
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Differential effects of TNF and LTalpha in the host defense against M. bovis BCG.

European journal of immunology·2001
Same author

Relationships between CD4 independence, neutralization sensitivity, and exposure of a CD4-induced epitope in a human immunodeficiency virus type 1 envelope protein.

Journal of virology·2001
Same author

The highly conserved C-terminal dileucine motif in the cytosolic domain of the human immunodeficiency virus type 1 envelope glycoprotein is critical for its association with the AP-1 clathrin adaptor [correction of adapter].

Journal of virology·2001
Same author

Long-term results in patients treated with posterior instrumentation and fusion for degenerative scoliosis of the lumbar spine.

Acta neurochirurgica·1999
Same author

A membrane-proximal tyrosine-based signal mediates internalization of the HIV-1 envelope glycoprotein via interaction with the AP-2 clathrin adaptor.

The Journal of biological chemistry·1998
Same author

[The effect of gamma rays on medazepam in a methanolic solution].

Pharmaceutica acta Helvetiae·1989
Same journal

Goodbye general animal safety tests.

Pharmeuropa bio & scientific notes·2026
Same journal

Establishment of a replacement batch for the Ph. Eur. Heparin low-molecular-mass for calibration CRS.

Pharmeuropa bio & scientific notes·2025
Same journal

Study for the Establishment of the Ph. Eur. Human Anti-D immunoglobulin Biological Reference Preparation batch 2

Pharmeuropa bio & scientific notes·2025
Same journal

Establishment of Erythropoietin for physicochemical tests CRS batch 2.

Pharmeuropa bio & scientific notes·2024
Same journal

Establishment of hepatitis A virus coating reagent batch 2 for <i>in vitro</i> potency assay of hepatitis A vaccines by ELISA.

Pharmeuropa bio & scientific notes·2024
Same journal

Collaborative study for the establishment of Golimumab Biological Reference Preparation Batch 1.

Pharmeuropa bio & scientific notes·2024
See all related articles

Related Experiment Video

Updated: May 15, 2026

Curtain Flow Column: Optimization of Efficiency and Sensitivity
06:44

Curtain Flow Column: Optimization of Efficiency and Sensitivity

Published on: June 12, 2016

A multifunctional test mixture for chiral cyclodextrin GC columns.

S Wyss1, I A Werner

  • 1Pharmaceutical Analytics, Institute of Pharmaceutical Science, ETH Zürich, Switzerland.

Pharmeuropa Bio & Scientific Notes
|January 19, 2013
PubMed
Summary
This summary is machine-generated.

A new chiral test mixture for cyclodextrin (CD) coated gas chromatography (GC) columns aids in selecting the best columns for chiral separations. This mixture helps monitor system performance and ensures reliable results in chiral analysis.

More Related Videos

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography
10:14

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

Published on: September 2, 2020

Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B
11:44

Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B

Published on: January 19, 2022

Related Experiment Videos

Last Updated: May 15, 2026

Curtain Flow Column: Optimization of Efficiency and Sensitivity
06:44

Curtain Flow Column: Optimization of Efficiency and Sensitivity

Published on: June 12, 2016

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography
10:14

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

Published on: September 2, 2020

Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B
11:44

Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B

Published on: January 19, 2022

Area of Science:

  • Analytical Chemistry
  • Separation Science
  • Chromatography

Background:

  • Gas chromatography (GC) is crucial for separating chiral compounds.
  • Selecting appropriate chiral stationary phases (CSPs) for cyclodextrin (CD)-coated GC columns can be challenging.
  • A standardized method for evaluating and comparing different CD-GC columns is needed.

Purpose of the Study:

  • To develop and introduce a novel chiral test mixture for evaluating CD-coated GC columns.
  • To provide a tool for comparing, selecting, and identifying the most suitable GC columns for specific chiral separation challenges.
  • To serve as a foundational aid for practitioners in chiral GC analysis.

Main Methods:

  • Development of a comprehensive chiral test mixture containing 12 enantiomer pairs.
  • The mixture encompasses a wide array of functional groups.
  • The test mixture is designed for compatibility with all modified cyclodextrin capillary GC columns.

Main Results:

  • The developed test mixture is the first of its kind suitable for all modified cyclodextrin capillary GC columns.
  • It enables effective comparison and selection of GC columns for diverse chiral separations.
  • The mixture facilitates monitoring of column performance and system changes, ensuring data reliability.

Conclusions:

  • This new chiral test mixture is a valuable tool for optimizing chiral separations using GC.
  • It simplifies column selection and performance monitoring, leading to more dependable analytical outcomes.
  • The mixture serves as an essential starting point for researchers and analysts in the field of chiral gas chromatography.