Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Analysis of the association between dietary patterns and nonalcoholic fatty liver disease in a county in Guangxi.

BMC gastroenterology·2023
Same author

ANGPTL2+cancer-associated fibroblasts and SPP1+macrophages are metastasis accelerators of colorectal cancer.

Frontiers in immunology·2023
Same author

Gastrointestinal Bleeding From a Transverse Colon Dieulafoy Lesion.

Cureus·2023
Same author

IGF2BP3 drives gallbladder cancer progression by m6A-modified CLDN4 and inducing macrophage immunosuppressive polarization.

Translational oncology·2023
Same author

ApoE gene polymorphisms and metals and their interactions with cognitive function.

BMC medical genomics·2023
Same author

Preliminary exploration of the effects of environmental factors on the microsatellite status of BRAF-mutated colorectal cancer.

World journal of surgical oncology·2023
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: May 15, 2026

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System
14:12

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Published on: November 21, 2023

Integrated Fluidic Circuits (IFCs) for digital PCR.

Ramesh Ramakrishnan1, Jian Qin, Robert C Jones

  • 1Fluidigm Corporation, South San Francisco, CA, USA. ramesh.ramakrishnan@fluidigm.com

Methods in Molecular Biology (Clifton, N.J.)
|January 19, 2013
PubMed
Summary
This summary is machine-generated.

The Fluidigm Digital Array integrated fluidic circuit (IFC) enables digital PCR by partitioning DNA molecules into nanoliter chambers for precise amplification and detection. This technology revolutionizes gene analysis, including copy number studies and rare mutation detection.

More Related Videos

Microfluidic Chip Fabrication and Method to Detect Influenza
09:43

Microfluidic Chip Fabrication and Method to Detect Influenza

Published on: March 26, 2013

A Microfluidic Chip for ICPMS Sample Introduction
11:16

A Microfluidic Chip for ICPMS Sample Introduction

Published on: March 5, 2015

Related Experiment Videos

Last Updated: May 15, 2026

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System
14:12

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Published on: November 21, 2023

Microfluidic Chip Fabrication and Method to Detect Influenza
09:43

Microfluidic Chip Fabrication and Method to Detect Influenza

Published on: March 26, 2013

A Microfluidic Chip for ICPMS Sample Introduction
11:16

A Microfluidic Chip for ICPMS Sample Introduction

Published on: March 5, 2015

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Nanotechnology

Background:

  • Digital PCR offers high precision for nucleic acid quantification.
  • Traditional digital PCR methods often rely on sample dilution.
  • Integrated Fluidic Circuits (IFC) provide a platform for miniaturized biological assays.

Purpose of the Study:

  • To introduce the Fluidigm Digital Array IFC as a novel platform for digital PCR.
  • To highlight the advantages of partitioning DNA molecules over dilution.
  • To showcase the applications of IFC technology in various genomic analyses.

Main Methods:

  • Utilizes a nanofluidic biochip with a network of fluid lines, NanoFlex™ valves, and reaction chambers.
  • Partitions individual DNA molecules into nanoliter-sized chambers for PCR amplification.
  • Employs fluorophore-containing probes for detecting amplified DNA (positive signal).

Main Results:

  • Achieves precise delivery of reagents and amplification of single DNA molecules.
  • Enables accurate absolute quantitation (molecule counting) of DNA and cDNA.
  • Facilitates sensitive detection of rare mutations and digital haplotyping.

Conclusions:

  • The Digital Array IFC represents a significant advancement in digital PCR technology.
  • IFC technology offers a simple, fast, and reagent-efficient method for molecular analysis.
  • This platform has broad applications in gene copy number studies, mutation detection, and quantitative genomics.