microFIND(®) approach to fluorescent in situ hybridization (FISH)

Area of Science:

• Molecular Cytogenetics
• Oncology
• Biotechnology

Background:

• Fluorescence in situ hybridization (FISH) is a key molecular cytogenetics technique for identifying genetic aberrations in cancer.
• Interphase FISH is routinely used for evaluating hemato-oncological and solid tumors, aiding in predictive and prognostic indications.
• Current FISH assays, while reliable, are limited by high reagent costs, hindering widespread oncological screening.

Purpose of the Study:

• To describe a significant advancement in interphase FISH technology for cytological samples.
• To introduce the miniaturized device microFIND® as a cost-effective solution for FISH assays.
• To highlight the potential for full automation of FISH technology.

Main Methods:

• Development and description of the miniaturized device microFIND® for interphase FISH.
• Focus on application for cytological samples.
• Exploration of cost reduction per assay.

Main Results:

• The microFIND® device offers a reduction in the cost per FISH assay.
• This miniaturization presents a novel approach to FISH technology.
• The device enables future full automation of FISH assays with robotic platforms.

Conclusions:

• The microFIND® device represents a major improvement in interphase FISH technology.
• It addresses the cost limitations of current FISH assays, making it more accessible.
• This innovation holds promise for expanding the use of FISH in large-scale oncological screening and diagnostics.