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Updated: May 15, 2026

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses
Published on: May 6, 2019
Evaluating CD8⁺ T cell responses in vitro
1Unite "Immunite Cellulaire Antivirale", Institut Pasteur, Paris, France. francois.lemonnier@inserm.fr.
Abstract:
The (51)Cr-release assay described in the 1960s has been for decades the gold standard cytolytic assay and remains in use in many laboratories. Whereas other radioactive tests were later on described, they never fully replaced the (51)Cr-release assay. More thorough understanding of CTL biology and killing pathways has more recently resulted in the design of reliable nonradioactive tests to analyze CD8(+) T cell responses which are likely to supplant in a close future the (51)Cr-release assay.

