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Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method
Published on: June 14, 2020
Chromatofocusing purification of CD1b-antigen complexes and their analysis by isoelectric focusing
Luis Fernando Garcia-Alles1, Henri de la Salle2
1Institut de Pharmacologie et de Biologie Structurale (IPBS), UMR 5089 CNRS/Université Toulouse III-Paul Sabatier, Toulouse, France. luis-fernando.garcia-alles@ipbs.fr.
Abstract:
The presentation of lipid antigens to T cells is mediated by the CD1 proteins. Purified functional CD1/lipid complexes are valuable tools to investigate such immune processes. Here, we describe how these complexes can be prepared in vitro, how they can be purified by chromatofocusing and how to control their antigen-loading status by isoelectric focusing.
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