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Updated: May 15, 2026
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Published on: March 24, 2022
Photodynamic therapy for pythiosis
Layla Pires1, Sandra de M G Bosco, Nelson F da Silva
1Physics Institute of São Carlos and Instituto de Biociências de Botucatu, Univ Estadual Paulista, Campus Botucatu, Departamento de Microbiologia e Imunologia, Distrito de Rubião Júnior, S/N, 18618-970, Botucatu-SP, Brazil. laylabtu@gmail.com
Photodynamic therapy (PDT) effectively inactivates Pythium insidiosum, the cause of pythiosis. This study demonstrated PDT
Area of Science:
- Medical Mycology
- Photobiology
- Infectious Diseases
Background:
- Pythiosis is a severe infection caused by Pythium insidiosum.
- Photodynamic therapy (PDT) offers a potential alternative to surgical intervention for pythiosis.
Purpose of the Study:
- To assess the efficacy of PDT against Pythium insidiosum in vitro.
- To evaluate PDT's effectiveness in an in vivo pythiosis model.
Main Methods:
- In vitro and in vivo studies utilized two photosensitizers: a haematoporphyrin derivative and a chlorine.
- PDT involved laser irradiation (660 nm, 200 J/cm²) on P. insidiosum cultures and rabbit models.
- Comparative analysis included amphotericin B and saline solution controls.
Main Results:
- In vitro, PDT achieved 60–100% inhibition of P. insidiosum, outperforming amphotericin B.
- In vivo, histological examination of rabbit lesions revealed no infection up to 1 cm depth post-PDT.
Conclusions:
- PDT demonstrates significant efficacy in inactivating Pythium insidiosum.
- PDT presents a promising novel therapeutic strategy for treating pythiosis.
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