Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mitogenic activity from trout embryos.

P Collodi1, D W Barnes

  • 1Department of Biochemistry and Biophysics, Oregon State University, Corvallis 97331-6503.

Proceedings of the National Academy of Sciences of the United States of America
|May 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Characterization of human plasma growth inhibitory activity on serum-free mouse embryo cells.

In vitro cellular & developmental biology. Animal·2016
Same author

Distribution of fission products in animals at Maralinga.

Progress in nuclear energy. Series 6 Biological sciences·2014
Same author

Spontaneous and induced changes in cell populations in heavily irradiated mice.

Progress in nuclear energy. Series 6 Biological sciences·2014
Same author

Cell and molecular biology of the spiny dogfish Squalus acanthias and little skate Leucoraja erinacea: insights from in vitro cultured cells.

Journal of fish biology·2012
Same author

Culture of cells from two life stages of Schistosoma mansoni.

Cytotechnology·2012
Same author

Cell cultures derived from early zebrafish embryos differentiate in vitro into neurons and astrocytes.

Cytotechnology·2012
Same journal

Chemotactic self-organization captures the dynamics of mammalian hair follicle patterning.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Tomographic imaging of superconducting order using particle-hole interference.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Inhibitory potential of autologous neutralizing antibodies sets quantitative limits on the rebound-competent HIV-1 reservoir.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Inferring epidemiological parameters under an infectious phylogeography model with visitor dynamics.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Analytical modeling for suction cup designs for skin-interfaced wearable devices.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Improving cell-free metabolism through direct integration of artificial respiratory chains.

Proceedings of the National Academy of Sciences of the United States of America·2026
See all related articles

Rainbow trout embryo extract promotes growth in fish cell lines without serum. This extract may contain novel growth factors for aquatic species, offering potential for cell culture advancements.

Area of Science:

  • Aquaculture
  • Cell Biology
  • Fish Physiology

Background:

  • Serum-free cell culture is crucial for reducing costs and contamination risks in fish cell line maintenance.
  • Identifying effective growth-promoting substances is essential for optimizing cell culture conditions for various fish species.

Purpose of the Study:

  • To investigate the growth-promoting potential of rainbow trout embryo extract on piscine cell lines.
  • To determine the efficacy of the extract in supporting long-term, serum-free cell culture.

Main Methods:

  • Rainbow trout embryo extract was prepared and applied to established fish cell lines (RTG-2, STE-137, CHSE-214, EPC, CAR) and primary cells.
  • Cell growth and proliferation were assessed in serum-free media supplemented with varying concentrations of the extract.

Related Experiment Videos

  • Characterization of the extract's activity involved testing its stability and response to proteases and mammalian growth factors.

    • Main Results:

    • The extract significantly stimulated the growth of multiple fish cell lines and early-passage cells in serum-free conditions.
    • Long-term cultures (20+ passages) of CHSE-214 cells were successfully maintained for months without serum.
    • Optimal growth promotion occurred at 100 µg/mL, with significant effects observed as low as 2.5 µg/mL. The activity was protease-sensitive and stable in acetic acid.
    • The extract's mitogenic effect could not be replicated by mammalian growth factors and did not affect mouse fibroblasts.

    Conclusions:

    • Rainbow trout embryo extract contains potent, novel growth-promoting activity for fish cells.
    • This extract offers a promising alternative to serum for maintaining and propagating fish cell lines, potentially reducing costs and improving culture efficiency.
    • Further research into the specific components and mechanisms of this activity is warranted.