Five new phenolic glycosides from Hedyotis scandens

Area of Science:

• Natural Product Chemistry
• Pharmacology
• Virology

Background:

• Hedyotis scandens is a plant source of bioactive compounds.
• Phenolic glycosides and cyanogenic glycosides are classes of natural products with diverse biological activities.
• Respiratory Syncytial Virus (RSV) is a significant respiratory pathogen causing illness in all age groups.

Purpose of the Study:

• To isolate and characterize new compounds from Hedyotis scandens.
• To evaluate the in vitro antiviral activity of isolated compounds against RSV.

Main Methods:

• Isolation of compounds using chromatographic techniques.
• Structure elucidation of new compounds via spectroscopic analyses (NMR, MS) and acid hydrolysis.
• Antiviral activity assay using the cytopathic effect (CPE) reduction method.

Main Results:

• Five new phenolic glycosides, hedyotosides A-E (1-5), including a novel cyanogenic glycoside (1), were identified.
• Ten known compounds (6-15) were also isolated and identified.
• Compounds 6 and 15 exhibited anti-RSV activity with IC50 values of 20 μg/mL and 25 μg/mL, respectively.

Conclusions:

• Hedyotis scandens is a rich source of diverse phenolic glycosides.
• Compounds 6 and 15 possess significant in vitro antiviral properties against RSV.
• Further investigation into these compounds may lead to novel antiviral therapeutics.