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Mitochondrial metabolism transition cooperates with nuclear reprogramming during induced pluripotent stem cell
Wenbo Liu1, Qi Long, Keshi Chen
1Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, PR China.
Biochemical and Biophysical Research Communications
|January 22, 2013
Summary
Mitochondrial changes, including cristae remodeling, are crucial for generating induced pluripotent stem cells (iPSCs). Enhancing oxidative phosphorylation and lactic acid levels boosts iPSC generation efficiency.
Area of Science:
- Cell Biology
- Stem Cell Biology
- Mitochondrial Biology
Background:
- Induced pluripotent stem cells (iPSCs) are vital for regenerative medicine.
- Reprogramming mechanisms primarily focus on the cell nucleus, leaving mitochondrial roles unclear.
- Mitochondria are key organelles whose function in nuclear reprogramming requires investigation.
Purpose of the Study:
- To elucidate the role of mitochondrial metabolism transition in nuclear reprogramming.
- To investigate the impact of mitochondrial cristae remodeling on iPSC generation.
- To determine how mitochondrial inner membrane protein (IMMT) affects reprogramming efficiency.
Main Methods:
- Analyzing mitochondrial cristae morphology in iPSCs.
- Down-regulating IMMT to assess its effect on iPSC generation efficiency.
- Comparing reprogramming efficiency in cells with enhanced oxidative phosphorylation (OXPHOS) versus normal cells.
- Evaluating the effect of lactic acid on iPSC generation.
Main Results:
- Mitochondrial cristae remodeling was observed in iPSCs.
- Down-regulation of IMMT significantly reduced iPSC generation efficiency.
- Cells with an OXPHOS advantage exhibited higher reprogramming efficiency.
- Lactic acid, a glycolysis intermediate, enhanced iPSC generation.
Conclusions:
- Mitochondrial cristae remodeling is coupled with iPSC generation.
- Mitochondrial metabolism transition plays a significant role in nuclear reprogramming.
- Targeting mitochondrial function could be a strategy to improve iPSC generation for regenerative medicine.
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