Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

T1 Microscopic Oropharyngeal Carcinoma: Revealing the Unknown Primary Through Diagnostic TORS.

Head & neck·2026
Same author

Muscle mass, not fat mass, predicts vertebral fracture risk: Vietnam osteoporosis study.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2026
Same author

Easy access to Fe<sub>2</sub>N nanomaterials from Fe nanocrystals and investigation of their electrocatalytic properties for the water electrolysis and CO<sub>2</sub> reduction.

RSC advances·2025
Same author

3D printed nerve guidance conduit for biologics-free nerve regeneration and vascular integration.

Bioengineering & translational medicine·2025
Same author

3D Printed Nerve Guidance Conduit for Biologics-Free Nerve Regeneration and Vascular Integration.

bioRxiv : the preprint server for biology·2025
Same author

Intraoperative nerve-specific fluorescence visualization in head and neck surgery: a Phase 1 trial.

Nature communications·2025
Same journal

Bridging the Gap - Advancing Microfluidics From Laboratory to Point-of-Care.

IEEE reviews in biomedical engineering·2026
Same journal

Review of Current Advances in Ultrasound Computed Tomography for Medical Imaging.

IEEE reviews in biomedical engineering·2026
Same journal

Gas Embolism: Fundamentals, Diagnosis, and Treatment.

IEEE reviews in biomedical engineering·2026
Same journal

Sonogenetics for Precision Medicine: A Focus on Immunoengineering and Genome Engineering.

IEEE reviews in biomedical engineering·2026
Same journal

Current Trends in Ultrasound Wearables: Spotlight on System Architecture.

IEEE reviews in biomedical engineering·2026
Same journal

A Perspective on Non-Invasive Blood Pressure Monitoring: Bridging Emerging Principles, Enabling Technologies and Extended Applications.

IEEE reviews in biomedical engineering·2026
See all related articles

Related Experiment Video

Updated: May 15, 2026

Multispectral Real-time Fluorescence Imaging for Intraoperative Detection of the Sentinel Lymph Node in Gynecologic Oncology
06:37

Multispectral Real-time Fluorescence Imaging for Intraoperative Detection of the Sentinel Lymph Node in Gynecologic Oncology

Published on: October 20, 2010

Fluorescence imaging in surgery.

Ryan K Orosco1, Roger Y Tsien, Quyen T Nguyen

  • 1Division of Head and Neck Surgery, University of California San Diego, La Jolla, CA 92093-0647, USA.

IEEE Reviews in Biomedical Engineering
|January 22, 2013
PubMed
Summary
This summary is machine-generated.

Future surgery will utilize targeted fluorescence imaging probes to enhance surgeon vision. These "smart probes" offer real-time, high-contrast visualization of tissues, improving patient outcomes and reducing costs.

More Related Videos

Tissue-simulating Phantoms for Assessing Potential Near-infrared Fluorescence Imaging Applications in Breast Cancer Surgery
11:05

Tissue-simulating Phantoms for Assessing Potential Near-infrared Fluorescence Imaging Applications in Breast Cancer Surgery

Published on: September 19, 2014

A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting
08:57

A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting

Published on: March 9, 2017

Related Experiment Videos

Last Updated: May 15, 2026

Multispectral Real-time Fluorescence Imaging for Intraoperative Detection of the Sentinel Lymph Node in Gynecologic Oncology
06:37

Multispectral Real-time Fluorescence Imaging for Intraoperative Detection of the Sentinel Lymph Node in Gynecologic Oncology

Published on: October 20, 2010

Tissue-simulating Phantoms for Assessing Potential Near-infrared Fluorescence Imaging Applications in Breast Cancer Surgery
11:05

Tissue-simulating Phantoms for Assessing Potential Near-infrared Fluorescence Imaging Applications in Breast Cancer Surgery

Published on: September 19, 2014

A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting
08:57

A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting

Published on: March 9, 2017

Area of Science:

  • Surgical innovation
  • Medical imaging
  • Molecular diagnostics

Background:

  • Current surgical visualization relies on white-light reflectance, limiting tissue contrast and differentiation.
  • Distinguishing healthy, varying, and diseased tissues is challenging due to limited color palettes and dynamic range.
  • Technological advancements are needed to overcome the limitations of traditional surgical sight.

Purpose of the Study:

  • To review current and future applications of fluorescence imaging in surgery.
  • To highlight the role of targeted fluorescence imaging probes in enhancing surgical vision.
  • To explore innovations in image processing and instrumentation for fluorescence-guided surgery.

Main Methods:

  • Overview of fluorescence imaging principles and probe development.
  • Discussion of intraoperative applications in various surgical procedures.
  • Analysis of image processing techniques and advanced instrumentation.

Main Results:

  • Fluorescence imaging provides pseudo-color, high-contrast delineation of normal and pathologic tissues.
  • Targeted probes expand the visible spectrum, offering real-time molecular guidance.
  • Potential for improved surgical precision and diagnostic accuracy.

Conclusions:

  • Fluorescence imaging represents a paradigm shift in surgical visualization.
  • Smart probes promise to significantly improve patient safety and clinical outcomes.
  • This technology has the potential to reduce overall healthcare costs through enhanced efficiency.