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Fetal liver cell transplantation in scid mice
K Sanhadji1, M S Negrier, J L Touraine
1Transplantation and Immunobiology Laboratory, Hôpital Edouard Herriot, Lyon, France.
Abstract:
Mice with severe combined immunodeficiency (CB 17 scid) received isogeneic and allogeneic fetal liver cell transplantation. Immunological reconstitution was followed by immunoglobulin production, mitogen-induced proliferation, spleen and thymus lymphoid recolonization. Scid mice injected with isogeneic fetal liver cells showed normal IgM, IgG production and subnormal Con-A induced proliferation. Lymphoid organs were gradually repopulated. Mice having received allogeneic stem cells presented normal IgM and IgG secretion. They still had no mitogen-induced lymphocyte stimulation, two months after fetal cell transplantation, despite satisfactory repopulation of spleen and thymus. Reconstitution of cell-mediated immunity may be somewhat slower following allogeneic than isogeneic stem cell transplantation.