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Updated: May 15, 2026

Reconstitution of a Kv Channel into Lipid Membranes for Structural and Functional Studies
Published on: July 13, 2013
Reconstitution of functionally efficient SecA-dependent protein-conducting channels: transformation of low-affinity
Ying-hsin Hsieh1, Hao Zhang, Hongyun Wang
1Department of Biology and Center of Biotechnology and Drug Design, Georgia State University, Atlanta, GA 30303, USA.
Abstract:
Previous work showed that SecA alone can promote protein translocation and ion-channel activity in liposomes, and that SecYEG increases efficiency as well as signal peptide specificity. We now report that SecDF·YajC further increases translocation and ion-channel activity. These activities of reconstituted SecA-SecYEG-SecDF·YajC-liposome are almost the same as those of native membranes, indicating the transformation of reconstituted functional high-affinity protein-conducting channels from the low-affinity SecA-channels.
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