Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Antibody Structure01:10

Antibody Structure

Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mechanochemical Sulfur-Phenolate Exchange Click Reactions.

The Journal of organic chemistry·2026
Same author

Bimetallic-Node-Occupied MOF With Glycoside Hydrolase Activity for Efficient Bacterial Biofilm Hydrolysis.

Angewandte Chemie (International ed. in English)·2026
Same author

Electrochemical and Hydrolytic Stability of Bidentate Alkyne-Based Self-Assembled Monolayers on Gold: Effect of Head and Foot Chain Lengths.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Rhodo-Box: A Synthetic Biology Toolbox to Facilitate Metabolic Engineering of <i>Rhodobacter sphaeroides</i>.

ACS synthetic biology·2026
Same author

Regioselective Sesquiterpene Hydroxylation Directed by Tunnel Remodeling in Rieske Oxygenases.

JACS Au·2026
Same author

Rapid analysis of terpenes produced by fermentation using flow injection analysis coupled to APCI MS.

Analytical methods : advancing methods and applications·2026

Related Experiment Video

Updated: May 15, 2026

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms
15:27

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

Published on: April 17, 2017

Antibody orientation on biosensor surfaces: a minireview.

Anke K Trilling1, Jules Beekwilder, Han Zuilhof

  • 1Plant Research International, Wageningen, The Netherlands.

The Analyst
|January 23, 2013
PubMed
Summary
This summary is machine-generated.

This review covers antibody immobilization for biosensors. It discusses methods for attaching antibodies to surfaces, focusing on techniques for oriented antibody coupling to enhance biosensor performance.

More Related Videos

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications
14:43

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications

Published on: September 23, 2013

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors
07:22

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors

Published on: November 20, 2013

Related Experiment Videos

Last Updated: May 15, 2026

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms
15:27

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

Published on: April 17, 2017

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications
14:43

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications

Published on: September 23, 2013

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors
07:22

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors

Published on: November 20, 2013

Area of Science:

  • Biomedical Engineering
  • Surface Chemistry
  • Immunotechnology

Background:

  • Detection elements are crucial for analyte recognition in biosensors, requiring high specificity and binding affinity.
  • Antibodies (Abs) are increasingly utilized as detection elements, but their surface immobilization on biosensors presents a significant challenge.

Purpose of the Study:

  • To review recent advancements in the immobilization and surface study of antibodies for biosensor applications.
  • To explore various antibody species, immobilization techniques, and protein modification strategies for oriented antibody coupling.

Main Methods:

  • Introduction of antibody species used in biosensor detection.
  • Discussion of techniques for elucidating antibody orientation, including layer thickness and surface topology determination.
  • Presentation of non-covalent and covalent immobilization methods, distinguishing between oriented and randomly coupled antibodies.

Main Results:

  • Overview of diverse immobilization strategies, categorizing them by attachment type (non-covalent, covalent) and resulting antibody orientation (oriented, random).
  • Highlighting protein modification methods specifically applicable for achieving oriented antibody immobilization.

Conclusions:

  • Effective antibody immobilization is critical for optimizing biosensor functionality.
  • Recent techniques offer improved control over antibody orientation and surface presentation, paving the way for advanced biosensor designs.