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Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells
Published on: May 4, 2018
Fluoride modulates preosteoblasts viability and matrix metalloproteinases-2 and -9 activities
Camila Slompo1, Camila P Buzalaf, Carla A Damante
1Department of Biological Sciences, Bauru Dental School, USP - University of São Paulo, Bauru, SP, Brazil.
Brazilian Dental Journal
|January 23, 2013
Summary
Fluoride influences preosteoblast viability in a dose-dependent manner. Low fluoride doses may increase matrix metalloproteinase (MMP) activity, potentially regulating extracellular matrix remodeling.
Area of Science:
- Biochemistry
- Cell Biology
- Toxicology
Background:
- Preosteoblasts are crucial for bone formation.
- Matrix metalloproteinases (MMPs) play a key role in extracellular matrix remodeling.
- Understanding the effects of fluoride on these cells is important for bone health.
Purpose of the Study:
- To evaluate the impact of fluoride on preosteoblast viability.
- To assess the influence of fluoride on MMP-2 and MMP-9 activity.
- To investigate fluoride's effect on alkaline phosphatase (ALP) activity.
Main Methods:
- MC3T3-E1 murine preosteoblasts were cultured and treated with varying sodium fluoride (NaF) concentrations.
- Cell viability was assessed using MTT assay.
- MMP-2 and MMP-9 activities were measured by gel zymography, and ALP activity by colorimetry.
Main Results:
- High fluoride concentration (10(-3) M) for 96 hours reduced preosteoblast viability.
- Lower fluoride doses did not significantly alter cell viability or ALP activity.
- Low fluoride doses slightly increased MMP-2 and MMP-9 activities after 24 hours.
Conclusions:
- Fluoride modulates preosteoblast viability in a dose-dependent manner.
- Fluoride may regulate extracellular matrix remodeling by influencing MMP activity.
- Further research is needed to fully elucidate fluoride's role in bone metabolism.
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