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Effects of temporary vascular occluder poloxamer 407 gel on the endothelium
Arif Gucu1, Ilkin Cavusoglu, Onder Bozkurt
1Department of Cardiovascular Surgery, Bursa Yuksek Ihtisas Training and Research Hospital, Bursa, Turkey. dr.arifgucu@gmail.com
Background:
Coronary occlusion techniques during OPCAB may lead to an endothelial damage to the target vessel. The adverse effects of these techniques are well-known, and researches have been trying to find out new materials to occlude the coronary artery without an endothelial damage. In the present study, we investigate to the endothelial damage in the rat aorta which is occluded by Poloxamer 407 gel.
Methods:
Forty-five rats were randomized in three groups: (1) segment of the aorta was occluded with Poloxamer 407 gel in P 407 group; (2) segment of the aorta was occluded with microvascular clamp in MV clamp group; and (3) no onclusion was available in the Control group. The rats were sacrificed of observation, and a 15mm segment of the aorta was obtained as a specimen. Integrity of the endothelial lining was observed with a scanning electron microscopy.
Results:
Scanning electron microscopy revealed a statistically significant difference among the 3 groups (p<0,001) using the SPSS 13.0 test. No difference was found between the Control group and the P 407 group (p=0,059). The differences between MV clamp-Control group (p<0,001) and MV clamp-P 407 group were statistically significant (p<0,002).
Conclusions:
We suggest that Poloxamer 407 gel occlusion may be a safer and more effective method compared to the microvascular clamp occlusion.
Insights
Poloxamer 407 gel offers a safer alternative to microvascular clamps for coronary artery occlusion. This study found no endothelial damage with Poloxamer 407 gel, unlike traditional clamping methods.
Area of Science:
- Vascular Surgery
- Biomaterials Science
Background:
- Coronary artery occlusion techniques in Off-Pump Coronary Artery Bypass (OPCAB) surgery can cause endothelial damage.
- Research is ongoing to identify materials that can occlude coronary arteries without causing such damage.
Purpose of the Study:
- To investigate the potential of Poloxamer 407 gel to occlude the rat aorta without inducing endothelial damage.
- To compare the effects of Poloxamer 407 gel occlusion with microvascular clamp occlusion on the aortic endothelium.
Main Methods:
- Forty-five rats were divided into three groups: Poloxamer 407 gel occlusion (P 407 group), microvascular clamp occlusion (MV clamp group), and a control group with no occlusion.
- Aortic segments were analyzed using scanning electron microscopy to assess endothelial lining integrity.
Main Results:
- Scanning electron microscopy showed a statistically significant difference in endothelial integrity among the groups (p<0.001).
- No significant difference in endothelial integrity was observed between the Poloxamer 407 gel group and the control group (p=0.059).
- Significant endothelial damage was observed in the microvascular clamp group compared to both the control (p<0.001) and Poloxamer 407 gel groups (p<0.002).
Conclusions:
- Poloxamer 407 gel occlusion appears to be a safer method for coronary artery occlusion compared to microvascular clamps.
- The findings suggest Poloxamer 407 gel is a promising biomaterial for preventing endothelial damage during vascular occlusion procedures.
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