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Updated: May 14, 2026
Detection of Mitochondria Membrane Potential to Study CLIC4 Knockdown-induced HN4 Cell Apoptosis In Vitro
Published on: July 17, 2018
Turn-on fluorescence detection of apoptotic cells using a zinc(II)-dipicolylamine-functionalized poly(diacetylene)
Yong-Suk Cho1, Kyung Mi Kim, Duhwan Lee
1Department of Chemistry and Center for Electro-Photo Behaviors in Advanced Molecular Systems, Pohang University of Science and Technology, San 31 Hyoja-dong, Pohang 790-784, Korea.
Abstract:
A liposome-based fluorescence sensing system for apoptotic cells has been developed from stimuli-responsive poly(diacetylene)-liposomes for the first time. The combination of the liposome components, a phosphatidylserine-binding Zn(II)-dipicolylamine component and an alcohol-terminated component in the ratio of 2:1, has led to an efficient detection system for apoptotic cells, as demonstrated by confocal fluorescence microscopy and FACS analysis. The liposome shows a color change from blue to reddish purple and emits fluorescence in the turn-on mode upon interaction with phosphatidylserine. The present system thus avoids the washing steps required for "always-on"-type sensing systems.
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