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Updated: May 14, 2026

Analyzing Protein Dynamics Using Hydrogen Exchange Mass Spectrometry
Published on: November 29, 2013
Rapid characterization of hydrogen exchange in proteins
Anushikha Thakur1, Kousik Chandra, Abhinav Dubey
1NMR Research Centre, Indian Institute of Science, Bangalore-560012, India.
Abstract:
Kinetics and thermodynamics of amide hydrogen exchange in proteins can be investigated with two-dimensional (13)CO-(15)N NMR correlation experiments. The spectra are acquired with high resolution and sensitivity. A single type of experiment on one sample serves to characterize hydrogen-deuterium fractionation factors and hydrogen-exchange rates that span three orders of magnitude.
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