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Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry
Published on: June 26, 2018
Phenotypic correlations between monocytes and CD4+ T cells in allergic patients
Marcin Moniuszko1, Krzysztof Kowal, Marta Jeznach
1Departments of Allergology and Internal Medicine, Medical University of Bialystok, Bialystok, Poland. moniuszm@umb.edu.pl
International Archives of Allergy and Immunology
|January 25, 2013
Summary
Allergic rhinitis alters immune cells. Monocyte and CD4+ T cell phenotypes are linked in allergic patients, suggesting new therapeutic targets for immune system modulation.
Area of Science:
- Immunology
- Allergy Research
- Cellular Biology
Background:
- Innate and adaptive immune systems are crucial in allergic diseases.
- In vivo interactions between these systems in allergies require further investigation.
Purpose of the Study:
- To explore reciprocal phenotypic relationships between monocytes and CD4+ T cells in allergic rhinitis patients.
- To analyze correlations between specific cell surface markers and cytokine receptors on these immune cells.
Main Methods:
- Simultaneous flow cytometry was used to analyze monocytes and CD4+ T cells.
- 50 allergic rhinitis patients and 29 healthy controls were studied.
- Expression levels of CD14, CD16, CD163, CD206, CD124 (IL-4R), CD210 (IL-10R), CD25, CD127 (IL-7R), and ICOS were measured.
Main Results:
- In allergic individuals, monocyte CD163 expression correlated with IL-10R and CD206.
- Monocyte IL-10R expression correlated with CD206 and IL-4R in allergic patients, unlike controls.
- Monocyte IL-4R and IL-10R expression correlated with CD4+ T cell IL-4R and IL-10R in both groups.
Conclusions:
- Allergic rhinitis is associated with distinct phenotypic changes in circulating monocytes and CD4+ T cells.
- Reciprocal interactions between monocytes and CD4+ T cells are altered in allergic rhinitis.
- These findings highlight potential cellular targets for managing allergic diseases.
