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Pinching-off of Coated Vesicles

Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
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Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
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COP Coated Vesicles00:59

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Membrane-enclosed structures called vesicles transport proteins and lipids across the cell. The vesicles derive their cargo from the plasma membrane, Golgi, ER, or endosome. Coated vesicles are spherical, protein-coated carriers with a 50–100 nm diameter that mediate bidirectional transport between the ER and the Golgi. The distribution of proteins between the ER and Golgi complex is dynamic and is maintained by different coated vesicles. Their formation is driven by the assembly of different...
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Intralumenal Vesicles and Multivesicular Bodies

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Related Experiment Video

Updated: May 14, 2026

Reconstitution of a Kv Channel into Lipid Membranes for Structural and Functional Studies
10:22

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Published on: July 13, 2013

Kerr constant of vesicle-like droplets.

M Richterová1, V Lisý

  • 1Department of Biophysics, Institute of Physics, P.J. Šafárik University, Jesenná 5, 041 54 Košice, Slovakia.

Journal of Biological Physics
|January 25, 2013
PubMed
Summary

The Kerr effect on fluid droplets is analyzed, deriving the Kerr constant for droplets in electric fields. This research estimates the bending rigidity of droplet surface layers by comparing theoretical models with experimental data.

Keywords:
Kerr effectinterfacial elasticitymicroemulsion dropletspolarizabilityrefractive indicesspherical membranessurface bending rigiditiesvesicles

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Area of Science:

  • Physics
  • Colloid Science
  • Materials Science

Background:

  • The Kerr effect describes the optical properties of materials in response to an electric field.
  • Vesicle-like droplets, including cells and emulsion droplets, exhibit complex behaviors in electric fields.
  • Understanding droplet deformation and optical responses is crucial for various applications.

Purpose of the Study:

  • To derive and analyze the Kerr effect in dielectric fluid droplets immersed in another fluid.
  • To investigate the influence of droplet shape and membrane thickness on the Kerr constant.
  • To estimate the bending rigidity of the surface layer of microemulsion droplets.

Main Methods:

  • Theoretical derivation of the Kerr constant for a prolate ellipsoidal droplet in a weak electric field.
  • Evaluation of the Kerr constant for droplets with a membrane of non-zero thickness.
  • Comparison of theoretical predictions with existing experimental data on droplet microemulsions.

Main Results:

  • A theoretical framework for the Kerr effect in droplet systems was established.
  • The Kerr constant was calculated for both bare and membrane-covered droplets.
  • The bending rigidity of the surface layer was estimated by fitting theoretical models to experimental results.

Conclusions:

  • The Kerr effect provides a valuable tool for characterizing fluid droplets and their surface properties.
  • The derived theoretical models accurately describe the behavior of droplets in electric fields.
  • This study offers a method for estimating the bending rigidity of droplet interfaces.