Mechanism of spontaneous, concentration-dependent phospholipid transfer between bilayers

We have previously demonstrated that spontaneous phospholipid transfer between bilayer vesicles at higher vesicle concentrations is characterized not only by a first-order desorption rate but also by a second-order process dependent on vesicle concentration (Jones & Thompson, 1989b). We have extended our studies to examine the mechanism of this second-order process by investigating transfer as a function of lipid type, temperature, aqueous medium composition, and vesicle size. The results suggest a mechanism of concentration-dependent transfer in which the rate of lipid monomer desorption from vesicle bilayers is enhanced in transient vesicle-vesicle complexes.