Related Experiment Video
Updated: May 14, 2026
08:33
An Ex vivo Culture System to Study Thyroid Development
Published on: June 6, 2014
High-throughput sequencing will metamorphose the analysis of thyroid hormone receptor function during amphibian
Alexis G Grimaldi1, Nicolas Buisine, Patrice Bilesimo
1Laboratoire d'Evolution des Regulations Endocrinienne, Muséum National d'Histoire Naturelle, Département Régulation, Développement et Diversité Moléculaire, UMR 7221 CNRS, Paris, France.
Current Topics in Developmental Biology
|January 26, 2013
Summary
Thyroid hormone (TH) orchestrates amphibian metamorphosis via TH receptors (TR). New high-throughput technologies enable comprehensive analysis of TR
Area of Science:
- Developmental Biology
- Endocrinology
- Genomics
Background:
- Amphibian metamorphosis involves significant thyroid hormone (T3)-driven transformations.
- Thyroid hormone receptors (TR) are key regulators of gene expression during development.
- TR exhibit dual functions: repression without ligand and activation with ligand.
Purpose of the Study:
- To review the application of high-throughput technologies in amphibian metamorphosis research.
- To understand the diverse effects of T3 and the mechanism of TR action during metamorphosis.
Main Methods:
- High-throughput sequencing technologies
- Transcriptome analysis
- Functional genomic analysis
Main Results:
- TR's dual function model in premetamorphic and metamorphic stages.
- T3 binding to TR activates gene expression during metamorphosis.
- High-throughput technologies are revolutionizing the study of amphibian metamorphosis.
Conclusions:
- High-throughput technologies are essential for understanding complex developmental processes like amphibian metamorphosis.
- Further genomic and functional genomic analyses are needed to fully elucidate T3 effects and TR mechanisms.
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