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Related Concept Videos

Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
Susceptibility, Permittivity and Dielectric Constant01:26

Susceptibility, Permittivity and Dielectric Constant

When placed in an external electric field, a dielectric material gets polarized. The charge density in the dielectric material is given by the sum of the bound and free charge densities, while the total charge density can also be written in terms of the total electric field. The bound charge density can be measured in terms of polarization, leading to the relationship between electric displacement and polarization.
Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as  cells...

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Updated: May 14, 2026

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis
10:38

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Published on: September 3, 2013

A separability parameter for dielectrophoretic cell separation.

Ahmet C Sabuncu1, Ali Beskok

  • 1Department of Mechanical Engineering, Istanbul Technical University, Istanbul, Turkey.

Electrophoresis
|January 26, 2013
PubMed
Summary
This summary is machine-generated.

A new separability parameter optimizes dielectrophoresis (DEP) cell separation by analyzing cell Clausius-Mossotti (CM) factors. This method enhances the selection of operating conditions for separating cell pairs like Jurkat and B16 cells.

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Area of Science:

  • Biophysics
  • Cell Biology
  • Microfluidics

Background:

  • Dielectrophoresis (DEP) is a label-free cell separation technique.
  • Optimizing DEP parameters is crucial for efficient cell separation.
  • Accurate measurement of cell electrical properties is key to DEP optimization.

Purpose of the Study:

  • Introduce a novel separability parameter for selecting optimal DEP operating conditions.
  • Validate the parameter using specific cell types (Jurkat and B16 cells).
  • Correlate DEP separation efficiency with cell electrical properties.

Main Methods:

  • Defined a separability parameter based on Clausius-Mossotti (CM) factors.
  • Utilized microfluidic impedance spectroscopy to measure CM factors.
  • Generated separability maps and conducted cell separation experiments using interdigitated electrodes.
  • Analyzed impedance data with an equivalent circuit model to understand electrode polarization and energy allocation.

Main Results:

  • Demonstrated the effectiveness of the separability parameter in predicting optimal DEP conditions.
  • Successfully separated Jurkat and B16 cells based on CM factor differences.
  • Linked cell separation outcomes to impedance measurements and electrical property variations.

Conclusions:

  • The introduced separability parameter provides a robust method for optimizing DEP cell separation.
  • Microfluidic impedance spectroscopy is a valuable tool for characterizing cells for DEP applications.
  • Understanding electrode polarization effects is essential for accurate DEP manipulation and separation.