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RNA Stability

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...

RNA Stability

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...

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Types of RNA

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Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...

Types of RNA

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...

Types of RNA

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...

Types of RNA

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Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
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Related Experiment Video

Updated: May 14, 2026

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria

Published on: February 23, 2021

Small RNA modules confer different stabilities and interact differently with multiple targets.

José Marques Andrade¹, Vânia Pobre, Cecília Maria Arraiano

¹Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa Av. da República, Oeiras, Portugal.

|January 26, 2013

Summary

Bacterial small regulatory RNAs (sRNAs) like MicA control gene expression. Understanding MicA RNA structure, including its 5' domain, stem-loops, and 3' poly(U) tail, is key to modulating its stability and target interactions.

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Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

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Last Updated: May 14, 2026

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria

Published on: February 23, 2021

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

Area of Science:

Bacterial regulatory RNAs
RNA structure and function
Gene expression regulation

Background:

Bacterial small regulatory RNAs (sRNAs) regulate multiple target mRNAs, similar to animal microRNAs.
The MicA sRNA is known to repress genes encoding major outer membrane proteins.

Purpose of the Study:

To characterize RNA determinants governing MicA stability.
To analyze how these determinants influence MicA's target gene expression.
To understand the structural basis for MicA's regulatory functions.

Main Methods:

Site-directed mutagenesis to create altered MicA forms.
Comparison of stability and target regulation between wild-type and mutated MicA.
Analysis of RNase III and PNPase involvement in sRNA degradation.

Main Results:

The 5' domain influences MicA stability via RNase III.
Stem-loop 2 is crucial for MicA stability and abundance.
The 3' poly(U) tail is critical for MicA stability and affects all analyzed targets.
Different structural elements (5' domain, stem-loops, 3' tail) impact target binding.

Conclusions:

MicA's stability is modulated by its distinct structural regions ('sRNA anatomy').
MicA utilizes different modules for regulating diverse mRNA targets.
This knowledge enables engineering of non-coding RNAs for tailored target interactions.