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Related Concept Videos

Fixation and Sectioning01:03

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...

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Related Experiment Video

Updated: May 14, 2026

Protocol for HER2 FISH Using a Non-cross-linking, Formalin-free Tissue Fixative to Combine Advantages of Cryo-preservation and Formalin Fixation
10:21

Protocol for HER2 FISH Using a Non-cross-linking, Formalin-free Tissue Fixative to Combine Advantages of Cryo-preservation and Formalin Fixation

Published on: December 25, 2017

Rapid two-temperature formalin fixation.

David Chafin1, Abbey Theiss, Esteban Roberts

  • 1Ventana Medical Systems, Inc., Tucson, Arizona, USA.

Plos One
|January 26, 2013
PubMed
Summary
This summary is machine-generated.

This study introduces a novel formalin fixation protocol that improves speed and tissue quality for anatomic pathology. The new method enhances histomorphology and assay compatibility, including for phosphorylated epitopes in immunohistochemistry.

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Area of Science:

  • Anatomic Pathology
  • Histopathology
  • Biochemistry

Background:

  • Formalin fixation is critical for histopathology but lacks standardization, leading to preanalytical errors.
  • Current rapid fixation protocols may compromise histomorphology and assay performance.
  • Immunohistochemistry for phosphorylated epitopes is challenging with standard formalin fixation.

Purpose of the Study:

  • To develop an improved formalin fixation protocol addressing standardization, speed, and assay compatibility.
  • To leverage basic formalin biochemistry for protocol innovation.
  • To enhance tissue suitability for a wider range of downstream assays.

Main Methods:

  • Investigated formalin biochemistry to inform protocol design.
  • Developed a novel protocol involving subambient temperature pre-incubation followed by heated formalin exposure.
  • Compared the new protocol against standard methods regarding speed, morphology, and assay performance.

Main Results:

  • The novel protocol is faster than standard formalin fixation.
  • Histomorphology is preserved with the new protocol.
  • Tissue processed with the new method shows compatibility with an expanded range of assays, including phospho-epitope immunohistochemistry.

Conclusions:

  • The developed formalin fixation protocol offers a faster alternative to standard methods.
  • This protocol preserves essential histomorphology and improves tissue compatibility for advanced molecular assays.
  • The findings suggest a significant improvement for anatomic pathology workflows and diagnostic capabilities.