Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Transcription01:10

Transcription

Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription01:17

Transcription

Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in correctly synthesizing messenger RNA (mRNA). Transcriptional regulation is responsible for the differentiation of different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Semiautomated Production of Cell-Free Biosensors.

ACS synthetic biology·2025
Same author

Evaluating the persistence and stability of a DNA-barcoded microbial system in a mock home environment.

Synthetic biology (Oxford, England)·2022
Same author

Development of an activation tagging system for maize.

Plant direct·2019
Same author

Publisher Correction: Exome sequencing highlights the role of wild-relative introgression in shaping the adaptive landscape of the wheat genome.

Nature genetics·2019
Same author

Exome sequencing highlights the role of wild-relative introgression in shaping the adaptive landscape of the wheat genome.

Nature genetics·2019
Same author

Developing Transgenic Agronomic Traits for Crops: Targets, Methods, and Challenges.

Methods in molecular biology (Clifton, N.J.)·2018
Same journal

Coordinated changes in phloem speed and carbon allocation losses maintain phloem carbon dynamics in wheat leaves during drought.

Plant science : an international journal of experimental plant biology·2026
Same journal

Vascular bundle adaptation to water-salt stress in sweet sorghum: An integrated anatomical and physiological pathway analysis.

Plant science : an international journal of experimental plant biology·2026
Same journal

Combined T-DNA and CRISPR/Cas9 mutagenesis reveals redundant developmental roles of the Arabidopsis BAG family.

Plant science : an international journal of experimental plant biology·2026
Same journal

Systemic metabolic reprogramming underlies cold stratification-induced seed dormancy release in Cercis chinensis revealed by integrated physiological and multi-omics analyses.

Plant science : an international journal of experimental plant biology·2026
Same journal

Genome-wide characterization of phytosulfokine (PSK) genes in Populus trichocarpa reveals the roles of PtrPSK7/8 in root development.

Plant science : an international journal of experimental plant biology·2026
Same journal

OsGAPC3 modulates starch synthesis and grain chalkiness by integrating glycolysis and transcriptional regulation in rice.

Plant science : an international journal of experimental plant biology·2026
See all related articles

Related Experiment Video

Updated: May 14, 2026

Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters
09:22

Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters

Published on: November 26, 2013

Designed transcriptional regulators for trait development.

Joseph F Petolino1, John P Davies

  • 1Dow AgroSciences, 9330 Zionsville Rd., Indianapolis, IN 46268, USA. jfpetolino@dow.com

Plant Science : an International Journal of Experimental Plant Biology
|January 29, 2013
PubMed
Summary
This summary is machine-generated.

Precisely controlling gene expression is key for plant trait development. Designed transcriptional regulators offer targeted gene modulation for agricultural applications.

More Related Videos

Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences
11:25

Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences

Published on: February 11, 2019

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
11:32

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

Published on: March 27, 2020

Related Experiment Videos

Last Updated: May 14, 2026

Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters
09:22

Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters

Published on: November 26, 2013

Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences
11:25

Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences

Published on: February 11, 2019

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
11:32

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

Published on: March 27, 2020

Area of Science:

  • Plant Molecular Biology
  • Gene Regulation
  • Developmental Biology

Background:

  • Gene expression, particularly at the transcriptional level, is a primary driver of organismal development.
  • Complex hierarchical interactions govern regulatory proteins, their encoding genes, and downstream targets.
  • Constitutive overexpression of regulatory genes in plants yields interesting phenotypes but also developmental issues.

Purpose of the Study:

  • To explore precise control over gene expression for agricultural trait development.
  • To investigate the potential of designed transcriptional regulators for targeted gene modulation.

Main Methods:

  • Utilizing designed transcriptional regulators, which fuse engineered DNA-binding proteins with activator or repressor domains.
  • Targeting the expression of genes encoding key regulatory proteins.

Main Results:

  • Designed transcriptional regulators enable selective modulation of endogenous gene expression.
  • Precisely controlling the magnitude, timing, and cellular localization of regulatory gene expression is achievable.

Conclusions:

  • Designed transcriptional regulators are powerful tools for modulating gene networks.
  • Targeted regulation of genes encoding key regulatory proteins offers a pathway for advanced trait development in plants.