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Human endogenous retrovirus-H insertion screening
Mehrab Guliyev1, Sibel Yilmaz, Kaniye Sahin
1Department of Molecular Biology and Genetics, Faculty of Science, Istanbul University, Vezneciler, Istanbul 34134, Turkey.
Molecular Medicine Reports
|January 30, 2013
Summary
Human endogenous retroviruses (HERVs) like HERV-H show insertion polymorphism in the human genome. This study analyzed HERV-H integration variations using the IRAP technique in diverse individuals.
Area of Science:
- Genomics
- Molecular Biology
- Human Genetics
Background:
- Endogenous retroviruses (ERVs) constitute a significant portion of the human genome.
- Human endogenous retrovirus (HERV)-H is a prominent family with implications for genomic variation.
Purpose of the Study:
- To analyze genome integration polymorphisms of the HERV-H family.
- To investigate variations using specific LTR sequences (LTR7A, LTR7B, LTR7C).
Main Methods:
- Utilized the inter-retrotransposon amplified polymorphism (IRAP) technique.
- Employed 3 primer pairs targeting HERV-H LTR regions.
- Analyzed genomic DNA from 20 individuals of diverse ethnic backgrounds.
Main Results:
- Observed insertion polymorphism patterns ranging from 0% to 87% across all subjects.
- Demonstrated significant integration variations at the genomic level for HERV-H.
Conclusions:
- HERV-H exhibits considerable insertion polymorphism within the human genome.
- These findings enhance understanding of HERV-H's role in human genomic variation.
